diff --git a/gnu/local.mk b/gnu/local.mk
index e936343918..41f42e588f 100644
--- a/gnu/local.mk
+++ b/gnu/local.mk
@@ -1188,7 +1188,6 @@ dist_patch_DATA =						\
   %D%/packages/patches/python-pycrypto-CVE-2013-7459.patch	\
   %D%/packages/patches/python2-pygobject-2-gi-info-type-error-domain.patch \
   %D%/packages/patches/python-pygpgme-fix-pinentry-tests.patch	\
-  %D%/packages/patches/python-pytest-pluggy-compat.patch	\
   %D%/packages/patches/python2-subprocess32-disable-input-test.patch	\
   %D%/packages/patches/python-unittest2-python3-compat.patch	\
   %D%/packages/patches/python-unittest2-remove-argparse.patch	\
diff --git a/gnu/packages/check.scm b/gnu/packages/check.scm
index 69fe278728..186f4ba09a 100644
--- a/gnu/packages/check.scm
+++ b/gnu/packages/check.scm
@@ -684,15 +684,14 @@ standard library.")
 (define-public python-pytest
   (package
     (name "python-pytest")
-    (version "4.3.0")
+    (version "4.3.1")
     (source
      (origin
        (method url-fetch)
        (uri (pypi-uri "pytest" version))
-       (patches (search-patches "python-pytest-pluggy-compat.patch"))
        (sha256
         (base32
-         "077gzimi9xiiyzpc3xjpb5yfgz038xkldg91mmbdvzr7z15isyh6"))))
+         "08q58ad8vaxyb57ll8m611yb4xwzzhp09kxafmyqrrps6cnalbjr"))))
     (build-system python-build-system)
     (arguments
      `(#:phases
diff --git a/gnu/packages/patches/python-pytest-pluggy-compat.patch b/gnu/packages/patches/python-pytest-pluggy-compat.patch
deleted file mode 100644
index ac33b862e6..0000000000
--- a/gnu/packages/patches/python-pytest-pluggy-compat.patch
+++ /dev/null
@@ -1,64 +0,0 @@
-Fix test failures when built against Pluggy 0.9.
-
-Taken from this upstream commit:
-https://github.com/pytest-dev/pytest/commit/a68f4fd2b9e99c82476d0e04ebcf561aeddbcb2e
-
-diff --git a/testing/test_assertion.py b/testing/test_assertion.py
-index b659233eb..e4fe56c6f 100644
---- a/testing/test_assertion.py
-+++ b/testing/test_assertion.py
-@@ -209,7 +209,7 @@ def load(self, require=True, *args, **kwargs):
-                     import spamplugin
-                     return spamplugin
- 
--            def iter_entry_points(name):
-+            def iter_entry_points(group, name=None):
-                 yield DummyEntryPoint()
- 
-             pkg_resources.iter_entry_points = iter_entry_points
-diff --git a/testing/test_config.py b/testing/test_config.py
-index f9f22a63e..1e29b83f1 100644
---- a/testing/test_config.py
-+++ b/testing/test_config.py
-@@ -514,8 +514,8 @@ def test_preparse_ordering_with_setuptools(testdir, monkeypatch):
-     pkg_resources = pytest.importorskip("pkg_resources")
-     monkeypatch.delenv("PYTEST_DISABLE_PLUGIN_AUTOLOAD", raising=False)
- 
--    def my_iter(name):
--        assert name == "pytest11"
-+    def my_iter(group, name=None):
-+        assert group == "pytest11"
- 
-         class Dist(object):
-             project_name = "spam"
-@@ -552,8 +552,8 @@ def test_setuptools_importerror_issue1479(testdir, monkeypatch):
-     pkg_resources = pytest.importorskip("pkg_resources")
-     monkeypatch.delenv("PYTEST_DISABLE_PLUGIN_AUTOLOAD", raising=False)
- 
--    def my_iter(name):
--        assert name == "pytest11"
-+    def my_iter(group, name=None):
-+        assert group == "pytest11"
- 
-         class Dist(object):
-             project_name = "spam"
-@@ -583,8 +583,8 @@ def test_plugin_preparse_prevents_setuptools_loading(testdir, monkeypatch, block
- 
-     plugin_module_placeholder = object()
- 
--    def my_iter(name):
--        assert name == "pytest11"
-+    def my_iter(group, name=None):
-+        assert group == "pytest11"
- 
-         class Dist(object):
-             project_name = "spam"
-@@ -621,7 +621,7 @@ def load(self):
- def test_disable_plugin_autoload(testdir, monkeypatch, parse_args, should_load):
-     pkg_resources = pytest.importorskip("pkg_resources")
- 
--    def my_iter(name):
-+    def my_iter(group, name=None):
-         raise AssertionError("Should not be called")
- 
-     class PseudoPlugin(object):